Journal: Human Vaccines & Immunotherapeutics

Article Title: Structure-based design of oligomeric receptor-binding domain (RBD) recombinant proteins as potent vaccine candidates against SARS-CoV-2

doi: 10.1080/21645515.2023.2174755

Figure Lengend Snippet: Expression and characterization of recombinant proteins. (a) Schematic diagram of RBD recombinant proteins. (b) the recombinant proteins were expressed in Sf 9-baculovirus expression system, and the expression of each RBD recombinant protein was detected by Western blot with anti-Strep tag II monoclonal antibody (1:3000). (c) Purified recombinant proteins were examined by reducing and non-reducing SDS-PAGE with Coomassie blue staining. NR, non-reducing; R, reducing. (d) Purified recombinant proteins were examined by analytical gel filtration with Tricorn 10/200 Superdex 200 HR. A representative elution curve is shown.

Article Snippet: The purified proteins were analyzed by reducing and non-reducing SDS-PAGE with Coomassie blue staining and were further analyzed by gel-filtration chromatography (SEC) using a Tricorn 10/200 Superdex 200 HR column (GE Healthcare).

Techniques: Expressing, Recombinant, Western Blot, Strep-tag, Purification, SDS Page, Staining, Filtration

Journal: Journal of Peptide Science

Article Title: An explorative study towards the chemical synthesis of the immunoglobulin G1 Fc CH3 domain

doi: 10.1002/psc.3126

Figure Lengend Snippet: Attempts of stepwise synthesis of [Met(O)‐432]‐IgG1‐Fc 398–450 ( 1 ). A, Assembly attempt on polystyrene‐divinylbenzene 2‐chlorotrityl resin preloaded with glycine (0.63 mmol/g). The RP‐HPLC profiles of the crude peptide acids with free N ‐terminus at cycle 22 (till Cys‐429) and 32 (till Ser‐419, 1a TRT ) were obtained by using method A. B, Assembly attempt on the polar and low‐loaded resin NovaSyn TGT (0.2 mmol/g Fmoc‐glycine) by using three pseudoproline dipeptides (in red). The RP‐HPLC profile of the Fmoc‐protected crude peptide acid at cycle 44 (till Ser‐407, 1a TGT+ψ ) was obtained by using method C with the Nucleosil C‐18 column (MALDI‐TOF‐MS peaks for M + H + and [M + 2H + ]/2. M calc. for C 242 H 352 N 62 O 69 S 2 : 5298.01 Da)

Article Snippet: Analytical RP‐HPLC was performed using a Thermo Fisher Scientific Dionex UltiMate 3000 UHPLC system (Germering, Germany) and either a Syncronics C‐18 column (100 Å, 5 μm, 250 × 4.6 mm, Thermo Fisher Scientific) at a flow rate of 1.5 mL/min or a Nucleosil C‐18 column (100 Å, 5 μm, 250 × 4 mm, Macherey‐Nagel, Düren, Germany) at a flow rate of 1 mL/min.

Techniques: